Hepcidin / LEAP-1 (Human)
Liver-Expressed Antimicrobial Peptide 1 (Human)

4392-s 0.1 mg | 162.50 EUR

Asp – Thr – His – Phe – Pro – Ile – Cys – Ile – Phe – Cys – Cys – Gly – Cys – Cys – His – Arg – Ser – Lys – Cys – Gly – Met – Cys – Cys – Lys – Thr

Synthetic Product
(reported disulfide bonds between Cys⁷-Cys²³, Cys¹⁰-Cys¹³, Cys¹¹-Cys¹⁹ and Cys¹⁴-Cys²²

The purity is guaranteed to be higher than 99% by HPLC
Dose dependent anti-microbial activity of Hepcidin / LEAP-1 was confirmed by radial diffusion assay using E. coli.

Hepcidin / LEAP-1 (Human) is a Liver-Specific Antimicrobial Peptide / Iron-Regulatory Hormone

Novel Antimicrobial Peptides identified in Human Blood Ultrafiltrate

Antimicrobial peptides from plants and animals are classified into several groups or classes; these include the disulfide cross-linked peptides known as defensins [Nature, 475, 389 (2002)]. LEAP-1, a 25 amino acid residue peptide with antimicrobial activity containing 4 intramolecular disulfide linkages was recently identified in human blood ultrafiltrate. This new peptide LEAP-1, has now been included as a member of the disulfide cross-linked class of antimicrobial peptides [FEBS Lett., 480, 147 (2000)]. The same peptide named hepcidin-25 (Hepc) with 25 amino acid residues (Hepc25) together with the amino-terminally truncated peptides (Hepc22 and Hepc20) were isolated from human urine by another group [J. Biol. Chem., 276, 7806 (2001)]. The disulfide arrangement of this peptide has very recently been reported [J. Biol. Chem., 277, 37597 (2002)].

LEAP-1 was named after its characteristic feature as a „liver-expressed antimicrobial peptide“. Actually, this peptide exerts its antimicrobial effect against both Gram-positive and Gram-negative bacteria as well as yeast; for example, the IC50 value against Saccharomyces cerevisiae was 18 µM [FEBS Lett., 480, 147 (2000)]. This activity is salt-sensitive [J. Biol. Chem., 276, 7806 (2001)]. Gene expression of LEAP-1 was detected in extremely high levels in the liver, and to a significant but lesser extent in the heart and brain. No gene expression was observed in the kidney. Subsequently, supporting data has been accumulated showing that this peptide is involved in the regulation of iron homeostasis [Proc. Natl. Acad. Sci. U.S.A., 98, 8160 (2001)].

This multifunctional peptide, LEAP-1 / Hepcidin / Hepcidin-25, should be a useful tool in the clarification of the liver’s biological functions.

Shipping | Storage | Stability
Our product Hepcidin | LEAP-1 will be shipped at room temperature. Information about product specific storage conditions will be found on the vial. Stock solutions should be stored at -20°C. Please avoid repeated freezing and thawing by preparing aliquots. Hepcidin is very sensitiv against oxidation at the methionine residue, please use appropriate precautions.

References:

1. A. Krause, S. Neitz, H.-J. Maert, A. Schulz, W.-G. Forssmann, P. Schulz-Knappe, and K. Adermann, FEBS Lett., 480, 147 (2000) (Original; LEAP-1)
2. C.H. Park, E.V. Valore, A.J. Waring and T. Ganz, J. Biol. Chem., 276, 7806 (2001) (Original; Hepcidin)
3. H.N. Hunter, D.B. Fulton, T. Ganz and H.J. Vogel, J. Biol. Chem., 277, 37597 (2002) (S-S Bond)
4. R.E.Fleming and W.S. Sly, Proc. Natl. Acad. Sci. U.S.A., 98, 8160 (Hepcidin; Regulation of Iron Homeostasis)

Links to publications that use our peptide Hepcidin-25 (LEAP-1) | code 4392-s:

1. CE-MS method development for peptides analysis, especially hepcidin, an iron metabolism marker
2. Sustained plasma hepcidin suppression and iron elevation by Anticalin‐derived hepcidin antagonist in cynomolgus monkey

For Laboratory use only! Not for Drug Use!