RFamide Related Peptide-1 (Human)

RFamide Related Peptide-1 (Human)

105,00  excl. 19% VAT

SKU: 4380-s Category:

RFamide Related Peptide-1 (Human)
RFRP-1 (Human) | Prepro (56-92) Amide

4380-s 0.1 mg | 105.00 EUR

Ser – Leu – Asn – Phe – Glu – Glu – Leu – Lys – Asp – Trp – Gly – Pro – Lys – Asn – Val – Ile – Lys – Met – Ser – Thr – Pro – Ala – Val – Asn – Lys – Met – Pro – His – Ser – Phe – Ala – Asn – Leu – Pro – Leu – Arg – Phe – NH2

(M.W. 4256.9) C195H304N52O51S2

Synthetic Product

The purity is guaranteed to be higher than 99% by HPLC

Prolactin Secreting Peptide

Novel Neuropeptide with the carboxyl C-terminal Arg-Phe-NH2

Among peptides with the carboxyl (C)-terminal Arg-Phe-NH2 (RFamide family of peptides), FMRF-Amide code 4142 was first isolated from Mollusks in 1977 [Science, 197, 670 (1977)]. Another member of this family, Leu-Pro-Leu-Arg-Phe-NH2 code 4144, was identified in chicken brain in 1983, yet there had not been any reports of corresponding mammalian peptides with the same five C-terminal residues. Recently, a new related peptide was deduced from a human database search by elucidating its endogenous form through application of peptide/DNA chemistry. The name of the target peptide is RFamide-related peptide-1 (RFRP-1).

Human RFRP-1 was first predicted to be a 12 amino acid residue peptide. Subsequent synthesis and biochemical studies found it to be a ligand for the orphan receptor, OT7T022 (also called FF1) [Nat. Cell Biol., 2, 703 (2000) ]. Later, the endogenous form of human RFRP-1 was determined to be composed of 37 amino acid residues including the above 12-residue peptide at its C-terminus [Biochim. Biophys. Acta., 1540, 221 (2001) ]. The same peptide was isolated by another group who named it NPSF(1-37) [J. Biol. Chem., 276, 36961 (2001) ]. Human RFRP-1 inhibits forskolin-stimulated cAMP production in CHO cells expressing OT7T022 (ED50=21 nM). The potency is slightly less than that of the 12-residue peptide (ED50=4.9 nM), however, this result suggests that the C-terminal portion of RFRP-1 is responsible for the recognition of OT7T022. Immunoreactive RFRP-1 was observed in the central nervous system (CNS), with the highest amount detected in the hypothalamus. Specific receptors of RFRP-1, OT7T022 or FF1, have been found in the CNS. It is interesting to note that the synthetic peptide composed of 12 amino acid residues described above has shown prolactin-releasing activity at 10 nM [Nat. Cell Biol., 2, 703 (2000) ] and that rat NPSF(1-37) has the anti-opioid effect in two models of nociception at 1.0 nmol/rat [J. Biol. Chem., 276, 36961 (2001) ].

This new synthetic product RFRP-1 should serve as an important biochemical tool to further research investigations.


  1. S. Hinuma, Y. Shintani, S. Fukusumi, N. Iijima, Y. Matsumoto, M. Hosoya, R. Fujii, T. Watanabe, K. Kikuchi, Y. Terao, T. Yano, T. Yamamoto, Y. Kawamata, Y. Habata, M. Asada, C. Kitada, T. Kurokawa, H. Onda, O. Nishimura, M. Tanaka, Y. Ibata and M. Fujino, Nat. Cell Biol., 2, 703 (2000) (Original; cDNA & Pharmacol.)
  2. S. Fukusumi, Y. Habata, H. Yoshida, N. Iijima, Y. Kawamata, M. Hosoya, R. Fujii, S. Hinuma, C. Kitada, Y. Shintani, M. Suenaga, H. Onda, O. Nishimura, M. Tanaka, Y. Ibata and M. Fujino, Biochim. Biophys. Acta., 1540, 221 (2001) (Endogenous Form)